A GoldenBraid cloning system for synthetic biology in social amoebae

GOLDENBRAID 2.0: A comprehensive DNA assembly framework for Plant Synthetic Biology Authors

GoldenBraid 2.0: a comprehensive DNA assembly framework for plant synthetic biology.

Plant synthetic biology aims to apply engineering principles to plant genetic design. One strategic requirement of plant synthetic biology is the adoption of common standardized technologies that facilitate the construction of increasingly complex multigene structures at the DNA level while enabling the exchange of genetic building blocks among plant bioengineers. Here, we describe GoldenBraid ...

Synthetic Generators for Cloning Social Network Data

Synthetic social network generators are useful for a variety of purposes, including benchmarking algorithms, modeling human interactions within agent-based simulations, and debugging code. Despite the increased availability of social media data, collecting data directly from these networks is not always feasible due to privacy concerns. Often data access is restricted to “silos” of analysts wit...

Cloning of oxidosqualene cyclases from Maytenus ilicifolia for synthetic biology

Material and methods For this purpose, we adopted the strategy of RT-PCR using degenerated primers obtained from sequences of known plant OSC genes. Total RNA was extracted from leaves of M. ilicifolia and used in the synthesis of cDNA. The PCR amplification of core fragments of OSC genes was performed with partially degenerated primers designed to anneal to highly conserved regions among OSC g...

GoldenBraid: An Iterative Cloning System for Standardized Assembly of Reusable Genetic Modules

Synthetic Biology requires efficient and versatile DNA assembly systems to facilitate the building of new genetic modules/pathways from basic DNA parts in a standardized way. Here we present GoldenBraid (GB), a standardized assembly system based on type IIS restriction enzymes that allows the indefinite growth of reusable gene modules made of standardized DNA pieces. The GB system consists of a...